Two years have passed since Jackie Estacado used the power of The Darkness to kill the men responsible for the murder of his girlfriend. Despite trying to suppress this supernatural power, he could not forget the memory of Jenny's death, and now The Darkness wants to be unleashed.

A sudden and unjustified attack on Jackie's life marks the beginning of a full-scale mafia war, which seems to have been organized by some external force. The failed attempt paves the way for the return of the Darkness and sets Jackie on a journey through hell and worse, as he tries to unravel the mystery behind the attack and the true intentions of the Darkness.